LC-MS/MS method development and validation for the determination of antiviral drug in human plasma

The validated protein precipitation method was applied to estimate Darunavir in human plasma with Verapamil as an internal standard. analytical column selected Agilent, Zorbax, and XDB C18 (2.1 x 50 mm ID, 5 μm). temperature set at 30°C. mobile phase composition acetonitrile:2Mm Ammonium Formate 0.1% formic acid water (70:30 v/v). Source flow rate 0.120 mL/min without a split. An injection volume of 10 µL. were eluted 1.35 &1.13 min, total run time 3.0 min for each sample